Sentence examples for by introducing additional mutations from inspiring English sources

Higher affinity peptides are generated from these leads by introducing additional mutations and transferring the pepitides onto a lower valency format to allow the selection of peptides with affinities in the low micromolar to nanomolar range.

Somatic hypermutation during humoral immune response fine-tunes affinity for antigen by introducing additional mutations in the variable domain, further increasing the sequence variation and in turn expanding the sequence diversity within a clonotype.

The variants with multiple mutations were obtained by introducing additional mutation(s) in a given variant, using the overlap PCR workflow described above.

We applied stresses externally, by exposing strains to environmental changes, and internally, by introducing additional random mutations.

Attempts to introduce additional mutations to double bBR mutants resulted in either no or significantly low yields of protein.

Moreover, derivatization influences the ionization efficacies by introducing additional charges.

In the S168W-environment variant, the environment of the new tryptophan was engineered to mimic the environment of the catalytic tryptophan in VPs [ 17] by introducing seven additional mutations, resulting, among other changes, in the substitution of three bulky phenylalanine residues located at the protein surface near the engineered Trp.

PB1+ was produced by introducing the additional mutation E80Q to PB1.

Hoping to patch up the protein, researchers at Johns Hopkins University in Baltimore, Maryland, and Memorial Sloan-Kettering Cancer Center in New York City, took mutated p53 genes and introduced additional random mutations by a process called PCR mutagenesis.

For construction of double mutants we used pHT315Ab-D136N harboring a point mutation D136N as template to introduce additional point mutations as E129K or T143D.

We have also constructed a quadruple mutation by introducing an additional P223G mutation.