Sentence examples for by introducing a stop from inspiring English sources

We also generated a truncated version of YeaG by introducing a stop codon at amino acid position Y382 (pBAD18-yeaG Y382stop), which results in only the AAA+ domain being expressed.

The exon-1-17Q construct was generated by introducing a stop codon into the N171 construct using QuikChange®.

The soluble HA (sHA) gene was generated by introducing a stop codon upstream of the transmembrane domain by PCR (K528).

A second allele was generated by targeted mutation by introducing a stop codon just upstream of the transmembrane domain.

The untagged WT FBXO7 was obtained using the QuikChange site-directed mutagenesis kit (Stratagene) by introducing a stop codon in front of the V5-His tag.

In addition to analyzing the A-loop, we analyzed the C-terminal region by introducing a stop codon at position 609 or 588 (Figure 1C).

pCMV-myc-LC3 ΔC was generated from wild-type by introducing a premature stop codon using site directed mutagenesis (QuikChange II XL, Agilent) following the manufacturer's instructions.

The EGFP gene was mutated by introducing a premature stop codon, TAG or TAA (Figure 1C).

The authors develop a model system of TSC by introducing a premature stop codon in the zebrafish tsc2 gene (which encodes the protein tuberin).

To investigate the pathophysiology of the disease and therapeutic approaches in mice, we generated a mouse model by introducing a premature stop codon in the UGT1a1 gene, which results in an inactive enzyme.

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