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Viral escape was then simulated by introducing a point mutation into either of the target sites.
We sought to identify the molecular requirements for activation of Jak2V617F by introducing a point mutation in the FERM domain (Y114A), required for receptor binding.
"It's almost as though they've induced a mass psychosis in society by introducing a point of view that has no common sense and no bearing on any deeper feeling".
We have therefore chosen to generate a more accurate model of the human disease via a gene targeting approach, by introducing a point mutation into the endogenous promoter.
The SalI site near the start codon was removed by introducing a point mutation in the sequence of primer E150 (bold and underlined in Table 1).
Class I AroA, originally a glyphosate-sensitive enzyme, can be converted into its glyphosate-tolerant form by introducing a point mutation.
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Similarly, inactivation of cGKII by either introducing a point mutation in the catalytic domain (cGKII-D576A [21]) or by addition of a specific pharmacological blocker KT5823 abolished the inhibitory cGKII effect on WT HCN2 to a similar extent as the S641A mutation (Fig. 4H, sixth and seventh column).
It seems helpful to divide the design region into two zones by introducing a specified point as critical height (CH), denoted by (bar{z}_{text{C}}) in dimensionless format.
Moreover, comparing the community structure coming from network analysis with the one expected on the basis of external (economical, political, and social) considerations can enrich the evaluation by introducing a different point of view on the system under examination, not driven by 'human' considerations, but purely mathematical in nature.
By introducing a single point mutation in the interprotofilament interface of CcMreB (V118E), single protofilaments are formed as shown by negative stain EM.
At each evolutionary time-step of an SCPE simulation, a trial sequence is generated by introducing a random point mutation in the current coding DNA sequence.
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