Sentence examples for by introducing a mutation from inspiring English sources

First we start by introducing a mutation in the operator controlling the expression of Tet-ON.

Thr189 is usually autophosphorylated, but this modification can be abrogated by introducing a mutation at this site [9].

This often occurs by introducing a mutation that irreversibly inactivates one or more genes involved along the glycan assembly line.

In the first, at time 0, we assumed complete linkage disequilibrium of QTL-markers (by introducing a mutation in a single haplotype) and linkage equilibrium between markers.

However, integrase-defective lentivectors (IDLVs) can be produced by introducing a mutation into the integrase gene, which specifically prevents lentivector DNA integration [ 11].

Furthermore interference with the endogenous chaperone system by introducing a mutation to Hsp70 could exacerbate the pathological phenotype, confirming the notion that Hsp70 is critical for maintaining α-synuclein's folding state [ 81].

Traditionally a gene has been classified to encode a virulence factor by experimentally introducing a mutation into the protein of interest and determining whether virulence of the resultant mutant is reduced.

Further, the native structure was mutated by introducing a point mutation in the native RpoS protein at P193L (proline to leucine) and native TviE protein at H53Y (histidine to tyrosine) using FixPDB with the NOMAD-Ref server [ 85] and validated with the SPDB viewer [ 86].

In order to test the functional effect of the human specific amino acids changes, we first constructed two mutant hMCPH1s by introducing a backward mutation of changing the human-specific amino acid to an ancestral amino acid (Met96Thr) and introducing a random mutation (Met96Leu) at Site-96.

Destabilization can be achieved by introducing a packing mutation into the hydrophobic core of N. The role of the packing mutation, which is selected to be distant from the binding site, is to decrease the stability of N (i.e., lower Kunf) without compromising the intrinsic affinity (Ka) or specificity for the ligand.

Therefore the wild type ABAleon1.1 was mutated to abolish phosphatase activity of ABI1 by introducing a D413L mutation in the catalytic metal-binding site (ABAleon2.1; http://www.uniprot.org/uniprot/P49597). 10.7554/eLife.01739.003 Figure 1.