Sentence examples for by intracellular staining using from inspiring English sources
FoxP3 expression was detected by intracellular staining using anti-human FoxP3 staining set with FITC-conjugated rat anti-human FoxP3 Ab (eBioscience), according to the manufacturer instructions.
Then the cells were incubated with FITC-conjugated anti-CD4 and APC-conjugated anti-CD8 (RPA-T8) antibodies for surface staining followed by intracellular staining using PE-conjugated anti-mouse IFN-γ (4S.B3) antibody and FIX/PERM buffers (BD Pharmingen) according to the manufacturer's instructions.
We also evaluated IL-17A protein expression by intracellular staining using flow cytometry.
IL-13 production was determined by intracellular staining using phycoerythrin (PE -labeled anti-human IL-13 antibody (clone JES10-5A2; BD Biosciences, San Jose, CA, USA).
Foxp3 expression was detected by intracellular staining using an anti-mouse/rat Foxp3 antibody (FITC-conjugated, FJK-16s) and the Foxp3 staining kit (eBioscience).
Helios expression was measured by intracellular staining using an anti-mouse/human Helios antibody (eFluor 450-conjugated, 22F6, BioLegend) and the Foxp3 staining kit (eBioscience).
This was done by first staining WT DC-enriched splenocytes with antibodies to the cell surface markers CD11c and CD8 followed by anti-Bcl-G intracellular staining using our mAbs.
Therefore, we analysed the expression of TLR 3 and 9 in DC by intracellular staining and used surface staining to assess TLR 2 and 4 expression (Fig. 1B and C).
Four hours later, cells were permeabilized using 1% Saponin (Sigma) and IFN-γ expression was detected by intracellular cytokine staining using PE-conjugated anti-IFN-γ monoclonal antibody (BD Biosciences).
Cyclin D1 and cyclin E1 expression was measured by intracellular protein staining using monoclonal antibodies for human cyclin D1 and cyclin E1 (Santa Cruz Technologies, Santa Cruz, CA).
Cyclin D1 expression was measured by intracellular protein staining using mAb to human cyclin D1 (clone 5D4) and Cytofix/CytoPerm kit (BD Biosciences), according to the instructions of the manufacturer.
