High throughput genomic technologies, by simultaneously interrogating the expression levels of thousands of genes, offers the potential to identify new biomarkers for early detection, prognosis, targets for therapy and for reclassification of prostate tumors.
By interrogating the co-expression network and the literature, the Wnt signaling pathway modulated in rx3-/ mutants was extended to include dmrta2 [ 36], fezf2 [ 37], nr2e1 [ 38], sox1b [ 32], foxg1a [ 39] and emx3 [ 11, 40].
To further clarify the role of FGF and SHH on hairy2 expression, we started by interrogating the ability of FGF8 to induce hairy2 in the absence of the ZPA.
By interrogating the whole genome, sufficient sequence diversity will be detected to differentiate between individual strains.
Two color microarrays experiments were performed by using Agilent platforms; 45,220 probe sets were tested to interrogate the expression of 30,886 unique human genes.
To interrogate the expression of PPAR-γ target genes in the dataset, 87 genes known from the literature to be regulated by PPAR-γ were extracted from the microarray data.
Efforts have been made into exploring effective strategies to interrogate the expression and activation of kinases.
We also interrogated the expression pattern of several splicing activators, again, no obvious pattern was found.
The expression level of a single mRNA, defined as the signal, was determined by the MAS 5.0 software which uses a weighted average fluorescence intensity difference obtained among the 11 probe pairs that interrogate the expression of each individual gene.
