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Sp1 levels were quantified by intensity analysis and shown with relative levels in the cells to those in the mock-transfected cells.
The Sp1 levels were normalized to the actin levels at each time point, being quantified by intensity analysis with ImageJ software.
Images were recorded at intensity levels below saturation, estimated by intensity analysis module.
Proteins that showed significantly different peptide counts (p<0.05) were pooled together with the proteins that showed a significant change by intensity analysis, to report the final list of significant changes.
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However, significant intracellular fluorescence signal could be detected either by the fluorescence microscopy observation or by fluorescence intensity analysis in both PC3 and RAW264.7 cells 3 hrs after the addition of rhodamine B loaded NPs.
The method is applied to the forced response of two panels which are coupled by a stiffened panel and a comparison is made with (i) exact results obtained by using the dynamic stiffness method, (ii) results yielded by a conventional SEA approach, and (iii) results yielded by wave intensity analysis (WIA).
Subsequently, special attention is focused on the energy transmission and mechanism of the box-type structure by structural intensity analysis.
The stress states and deformation principles of driving drums including both bionic covering-layer and common covering-layer are obtained by static intensity analysis with Finite Element Analysis (FEA) software ANSYS.
The dissociation constants (Kd [kanamycin] = 78.8 nM, Kd [kanamycin B] = 84.5 nM, and Kd [tobramycin] = 103 nM) of the new aptamer were determined by fluorescence intensity analysis using 5′-fluorescein amidite (FAM) modification.
Both theoretical deviation and experimental result prove that optical intensity is time invariant when the laser operates in single longitudinal mode, the SLM operation can be approximately verified by optical intensity analysis, which is practical to analyze longitudinal mode in the application of engineering.
Density data were collected by pixel intensity analysis using Image Pro version 6.3 (Mediacybernetics, Bethesda, MD).
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