Sentence examples for by integrating the linearized from inspiring English sources
The TAL1, TKL1, RPE1, and RKI1 genes involved in the nonoxidative pentose phosphate pathway were overexpressed in a single colony isolated from the BSW1AT 150 h culture by integrating the linearized plasmid pJPPP3 [ 19] into the chromosome.
The critical forces are then obtained by the linearized stability theory.
To transform the wild-type strains 15Daub and W303a using pRS/pRSII plasmids, we used either 200 ng of integrating plasmid linearized by restriction at a unique site within the yeast-selectable prototrophic marker sequence or 50 ng of CEN/2μ plasmid.
The linearized Avrami kinetic model equation (Avrami 1940) is expressed by Eqs.
The linearized targeting vector was transfected into 129 ES cells by electroporation.
The linearized targeting vector was transfected into 129S6/SvEvTac-derived 129S6/SvEvTac-derived) cells by electroporation.
Linearized plasmids were transformed into an SMC1 or SMC3 shuffle strain to integrate the vector at the LEU2 locus and LEU+ clones isolated.
The PCR product was integrated into the parental plasmid, linearized with SmaI, by co-transformation and in vivo homologous recombination, resulting in an in-frame fusion to a triple FLAG/His10/HA2 (F/H) epitope affinity tag immediately upstream of the ORF.
Furthermore, the linear and linearized generalized semi-infinite optimization problems were introduced by Rukmann [14].
