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These results indicate that the HdrABC protein expressed from the integrated copy of the pJC1 plasmid is enzymatically active and that by integrating a second copy of the hdrABC* operon onto the chromosome it is possible to increase the level of HdrABC enzyme activity in cell extracts above wild-type levels.
To facilitate the inactivation of the mdh gene, a diploid strain was constructed by integrating a second copy of the gene at the attB site [50].
As an alternative approach, we increased the copy number of GAL80 in both the euploid EC33-gal and YPS163-gal parents by integrating a second copy of the gene (the well-characterized S288c allele) under its native promoter.
The new German Rectal Cancer Trial show a higher rate of complete remission without a significantly higher rate of toxicity by integrating a second drug in the neoadjuvant radiochemotherapy schedule [ 24].
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Limitations of both approaches are addressed by integrating a third camera to verify consistency of the stereo pairings and to reduce the complexity of the global selection.
The plasmid was further modified by integrating a BaeI site between the first and the second triplets of the ZeoR coding sequence.
Regarding the second aim, by integrating a WGOH descriptor with a DMELA-trained TNFN to form an image alignment system could estimate affine parameters accurately.
The previously proposed tracking-by-matching (TbM) solution [35] integrates a third camera to conduct projection consistency check into the probabilistic approach.
First, we present algorithms for both the above-mentioned paradigms (see Figure 1): The previously proposed tracking-by-matching (TbM) solution [35] integrates a third camera to conduct projection consistency check into the probabilistic approach.
In this study, we described a method to assemble the complete genome sequence of L. santarosai serovar Shermani strain LT821 de novo by integrating second- and third- generation sequencing methods with optical whole-genome mapping.
We further evaluated the strong signal in EGFR by integrating the second and third regions to form a haplotype spanning seven SNPs in intron 1 (Supplementary Table S2).
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