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In the remaining cases both alleles were observed by inspecting the alignment manually, but Maq failed to call both of the SNP alleles.
The leader sequences, framework regions, complementarity determining regions (CDRs) and J-regions were identified by inspecting the alignment of the F1-40 heand and light chains to other antibody sequences [26] [30].
The 3' and 5' splice sites were identified manually by inspecting the alignment and confirmed by comparing the genomic sequences to the corresponding exon sequences.
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The alignments were inspected and adjusted manually and are available upon request from K.F.O.
Identifying potential homologs by inspecting the alignments of the families takes an average of only 0.8 of the 4.7 seconds per query.
In the second stage, FastBLAST quickly finds the top homologs for a given gene by inspecting the alignments for the families that the query belongs to (both known families and ad hoc families).
The disrupted RM genes were reconfirmed by manually inspecting the alignment of the remaining parts.
Since the RefSeq annotations of chicken and zebrafish are incomplete, we identified additional orthologous exon pairs by projecting the human annotation on the other species' genome and confirming the orthology of the projected exons by inspecting the sequence alignment of the projected exons in the representative species.
Multiple sequence alignment viewers enable alignments to be visually reviewed, often by inspecting the quality of alignment for annotated functional sites on two or more sequences.
This was done by manually inspecting the alignments and the nucleotide sequences of the relevant duplicates.
By manually inspecting the alignments, we suspect that at least 42 of them are real AT-AC introns, while the others can be non-canonical ones.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com