Sentence examples for by inserting the sequence from inspiring English sources

Since we did not know which protease is involved in the cleavage of MsrB1, we engineered a cleavage site for factor Xa protease by inserting the sequence coding for tetrapeptide IEGR between Gly75 and Asn76 [13].

Chimeric human Notch receptors (Notch1, 2, and 3) were created by inserting the sequence encoding the DNA binding domain of Gal4 into a Notch cDNA previously deleted of the sequence encoding most of the RAM domain and the ankyrin repeat domain as described [35].

Type I transmembrane and GPI-linked proteins were N-terminally tagged with the FLAG epitope (DYKDDDDK) by inserting the sequence encoding FLAG into primers and using an overlapping PCR method to generate the full-length tagged construct as previously described [22].

The vector expressing neuropeptide Y (NPY) was generated by inserting the sequence encoding human pro-NPY into the pmRFP vector.

Plasmid pDCW174 (Table  1) was constructed by inserting the sequence of the CelA (Cbes1867) signal peptide into pDCW144 [ 27], which also contains the regulatory region of Cbes2303 and encodes a C-terminal 6X histidine-tag and a rho-independent transcription terminator.

Plasmids expressing GFP-GBP fusion proteins were obtained by inserting the sequences of GBP-1, -2 or -5 into the pMCV1.4-Flag-GFP vector using SnaBI and SalI restriction sites.

Construct a "dead" reporter plasmid by inserting the DNA sequence targeted by the TALENs' EBEs and the spacer sequence (~48 base pairs) into the GFP (green fluorescent protein) coding sequence.

A fragment containing all three UIM sequences from ataxin-3 (residues 194 361, UIM_123) was generated by inserting the corresponding sequence into the BamHI and XhoI sites of the pET21a vector (Novagen) that included an in-frame six-residue histidine tag.

The plasmid pAAV-CAG-GCamP6s18 was generated by inserting the CGaMP6s sequence of pGP-CMV-GCaMP6s (Plasmid #40753, Addgene) into the Nhe1/Not1 site of pAAV-CAG-Venus58 to replace the Venus sequence.

In order to bind the detected ranging codes with the estimated timing or frequency offsets, the methods either sacrifice the estimation performance by inserting the code sequence in the estimated parameters [14] or perform exhaustive full search by correlating all sets of ranging codes and offsets at the BS [11, 15].

Type II transmembrane proteins were C-terminally tagged by inserting the FLAG sequence upstream of the stop codon, thus adding the epitope tag to the ectodomain.