Sentence examples for by inserting the open from inspiring English sources

For overexpression of Neurog2, pEF1-Neurog2 was generated by inserting the open reading frame encoding Neurogenin2 into pEF1.

The GH RFP (red fluorescent protein) construct, encoding GH fused at its C-terminal end to the RFP, was made by inserting the open GH coding sequence into pTagRFP.

The GHR GFP (green fluorescent protein) construct, encoding the full-length GHR fused at its C-terminal end to the GFP, was generated by inserting the open GHR coding sequence into the pTagGFP vector (Evrogen).

The plasmid pZL204 was constructed by inserting the open reading frame of the sidCΔ100 sequence into SacI-BamHI site of pJB908 in which the region encoding the carboxyl terminal 100 amino acids of SidC was deleted and replaced with a premature stop codon.

Plasmid pEGFP-CuZnSOD (provided by Dr. BJ Park, Pusan National Univ., Korea) that was constructed by inserting the CuZnSOD open reading frame into plasmid pEGFP (Clontech, Mountain View, CA) were transfected into A549 cells using jetPEI (Polyplus transfection); stable cells were then selected with 0.5 mg/ml Geneticin (G418, GIBCO BRL) according to the manufacturer's instructions.

Plasmid pDCW180 (Fig.  1a; Additional file 1: Figure S1) was constructed by inserting the Teth39_0206 open reading frame into pDCW142 [ 26], which contains the regulatory region of Cbes2303, a C-terminal 6X Histidine-tag and a Rho-independent transcription terminator.

Plasmid pDCW171 was constructed by inserting the Teth39_1957 open reading frame into pDCW142 [ 11], which contains the regulatory region of Cbes2303, a C-terminal 6X Histidine-tag and a Rho-independent transcription terminator.

Oil red-O staining of KLF13 knockdown was performed at day 8. KLF13 overexpression vector (pcDNA3.1-KLF13 pcDNA3.1-KLF13 by inserting the wasle open readingeneratedORF) of porcine KLF13 into pcDNA3.1 (Invitrogen).

Transformation constructs for UAS-YC3.6, UAS-mito-DsRed, UAS-dp32, and UAS-dp32-EGFP transgenic lines were generated by inserting the respective open reading frame (ORF) for YC3.6 (Nagai et al. 2004), dP32 (cDNA accession number AY094810), or dP32 with EGFP fused to its C-terminus, into the P element transformation vector, pUAST (Brand and Perrimon 1993).

Similarly, vA28-HAi-Fire was created by inserting the firefly Luc open reading frame with the synthetic early/late promoter between the F12 and F13 open reading frames of vA28-HAi [52], a WR strain virus with an IPTG-regulated HA-tagged A28 protein.

A muscle-specific GCaMP-3 reporter plasmid was constructed by inserting the GCaMP-3 open reading frame (Tian et al., 2009) downstream of zebrafish mylz2 promoter sequences (von Hofsten et al., 2008).