Sentence examples for by inserting the human from inspiring English sources
By inserting the human gene coding for insulin into the E. coli cell, biotechnologists give this bacterium the ability to synthesize the hormone on an industrial scale.
He and his colleagues are also attempting to make an animal model of stuttering by inserting the human mutation into mice.
A reporter gene construct was made by inserting the human INS promoter into a luciferase expression plasmid (pGL4.10INS421bp; Fig. 2A).
The photoactivatable HP1α construct was created by inserting the human HP1α cDNA derived from pBCHGN-HP1α (18) cut with HindIII and BamH I into the HindIII and BglII of pPAGFP-C1 (19).
In summary, the NSe strain c-DNA infectious clone (derived from the MV-Edm vaccine lineage Seed B) [ 36] was engineered by inserting the human CEA gene upstream of the MV N gene.
17, 18 To establish HT1080-GFP cexpressingssing CEA HT1080-GFP-CEA HT1080-GFP-CEA HT1080-GFP-CEAby inserting the human CEA cDNA, cloned from mRNA of MKN45, into vector pcCAG, a modified version of vector pcDEF3 19 in wasconstructedα promoter is replaced by the CAG promoter.
The vector expressing neuropeptide Y (NPY) was generated by inserting the sequence encoding human pro-NPY into the pmRFP vector.
The protein-expression vector (pALTER-TUBB3) was prepared by inserting the full-length human class III β-tubulin cDNA fragment from pOTB7-TUBB3 (LGC Promochem) into pALTER Max (Promega, Southampton, UK).
However, something similar could probably be made by inserting the nucleus of a human cell into the egg of another species, such as a rabbit.
pGreen-FRS2 α, pGreen-Mcl-1 and pGreenPuro-vec constructs, encoding shRNA for FRS2 α (sh-FRS2), Mcl-1 (shMcl-1) or a negative control (vector) respectively, were prepared by inserting the target sequence for human FRS2 α (shRNA1: 5′-CCGTGATAGACATCGAGAGAA-3′ or shRNA2: 5′-CCGTGCAGAAGAATTATTT-3′) or Mcl-1 (5′-GGACTTTTATACCTGTTAT-3′) into pGreenPuro.
If Dr. Paabo and 454 Life Sciences should succeed in reconstructing the entire Neanderthal genome, it might in theory be possible to bring the species back from extinction by inserting the Neanderthal genome into a human egg and having volunteers bear Neanderthal infants.
