Sentence examples for by inserting the coding from inspiring English sources

The secretory expression plasmid pPIC9K-Rml was constructed by inserting the coding sequence of the R. miehei lipase gene (RML) into the vector pPIC9K (Invitrogen, USA).

Rog was made by inserting the coding sequence for emerald GFP Packardd) at the N-terminus of the receptor, after a FLAG tag (DYKDDDDV) and the first eight amino acids of the RASSL.

The pTrip-aOn plasmid was constructed by inserting the coding sequence of the Tet-On advanced transcriptional activator into pTrip-IRES-neo downstream from the elongation factor 1 alpha promoter.

A bacterial expression plasmid for the fusion of BTV-10 VproteineincorporatingiN-terminalnal hexahistidine tag (his-tag) was produced by inserting the coding region of S9 into the modified bacteria expression vector, pRSETA-imperial.

The plasmids for overexpression of EYFP, EYFP-D70-SF-1 and 3-FLAG-SF-1 were constructed by inserting the coding sequence of enhanced yellow fluorescent protein (EYFP), or wild-type or N terminal truncated (D70) SF-1 into pAS4w.1.Pbsd, which contains seven copies of modified tetO sequence.

In order to bind the detected ranging codes with the estimated timing or frequency offsets, the methods either sacrifice the estimation performance by inserting the code sequence in the estimated parameters [14] or perform exhaustive full search by correlating all sets of ranging codes and offsets at the BS [11, 15].

The attraction of phage display relies on the fact that a polypeptide can be displayed as fusion protein on the phage surface by inserting the gene coding for the polypeptide in the phage genome.

The GNL1-GFP construct was generated by inserting the GNL1 coding region into p068.

Transfer vectors were constructed by inserting the corresponding coding sequences into the polylinker of the pRRL-cPPT-CMV-X-PRE-SIN vector [46].

The Ntrab5-DsRed and ST-DsRed constructs were generated by inserting the GNL1 coding region and ST sequence into p067, respectively.

The pCI-EGFP plasmid (GFP fused to the C terminus of the targeted protein) was generated by inserting the EGFP coding sequence into Sal I and Not I sites of the pCI-neo vector (Promega).