Sentence examples for by inserting the chimeric from inspiring English sources

Moreover, transcriptional regulation could be rendered specific to human hepatocellular carcinoma (HCC) cells by inserting the chimeric gene Gal4/NF-YA under the regulation of the HCC-specific hybrid promoter.

Phrap [ 19] uses mate-pair information to flag potential chimeric clones by inserting the chimeric mate into a contig.

Chimeric human Notch receptors (Notch1, 2, and 3) were created by inserting the sequence encoding the DNA binding domain of Gal4 into a Notch cDNA previously deleted of the sequence encoding most of the RAM domain and the ankyrin repeat domain as described [35].

Plasmid quantification was carried out by inserting a chimeric nucleotide fragment into the pCR II plasmid (TA cloning Kit Dual Promoter (pCRII) Invitrogen, Carlsbad, CA, USA), as previously described [17].

The YBR194W-snR6 chimeric strain was generated by inserting a URA3 cassette at the 5′ end of the YBR194W gene in BY4741 ungΔ cells.

In order to obtain the chimeric construct, the gene fragment coding for the HP-RNase was cloned downstream to the Erb-hcAb sequence by inserting a spacer encoding an 11-amino-acid peptide linker (AAASGGPEGGS) between the scFv-Fc and the RNase.

A number of studies have confirmed the role of chimeric ORFs in male sterility by disrupting the function of ORFs by inserting or deleting a few base pairs (reviewed by Hanson and Bentolila).

Results reveal that the HTP may effectively be increased by inserting rarefied porous inserts.

This goal has been achieved by lentiviral transfection of two types of gene constructs: one is a monocistronic insert containing the chimeric tCD2-luc2 gene; the other one is a bicistronic insert resulting in concomitant expression of the wild-type luciferase 2 and the truncated rat CD2 as two distinct protein species.

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If chimeric nodes can be identified correctly, it means that the de Bruijn graph can be disconnected appropriately by splitting the chimeric nodes.