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In 2001 that company allowed subscribers to access master digital copies of music online after they had confirmed ownership by inserting the appropriate audio CD into their computer.
We generated siRNA expression plasmids that targeted PAPST1, PAPST2, NDST1, NDST2, and EGFP, a negative control, by inserting the appropriate dsDNAs between the BamHI and HindIII sites of pSilencer 3.1-H1 (Ambior) or pSUPER.retro.puro (OligoEngine).
Luciferase reporter constructs with classical HNF4α response elements (RE-1 and RE-2) were made by inserting the appropriate synthetic oligonucleotides into pGL4.23.
Model estimates for the various treatment conditions were calculated by inserting the appropriate mean values into the final models using untransformed parameters.
For expression in MDCK cysts, Dia-FL and Dia-ΔDAD were cloned into pEGFP-C1 by inserting the appropriate segments from a Dia cDNA using the SalI and HindIII sites.
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The plasmid pEGFP Vpr was constructed by inserting the Vpr gene amplified from the HIV-1 pNL4-3 clone into the appropriate vectors.
The problem was solved by inserting appropriate dyes in the emulsion.
Reducing hysteresis and creep in bimorph PZT X-ray optics is the only feasible way by inserting an appropriate capacitor in series with bimorph PZT optics to significantly reduce both effects.
Relations are being translated by inserting arrows with the appropriate style, which is given in the KGML document.
All KGFP variant constructs (see Fig. 2 and Results) were made by inserting appropriate synthetic linker DNAs (sequences available upon request) into the Bgl II – Xho I sites of KGFP and cloned into the Asp718 I – Xba I sites of the pCaSpeR4 vector for P element-mediated transformation.
Under the guidance of the alignment path, two DNA sequences are globally aligned by inserting blank spaces in the appropriate positions of these two sequences.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com