Sentence examples for by inserting sequences from from inspiring English sources
While bevacizumab is a full-length recombinant antibody, ranibizumab was developed by inserting sequences from the parent antibody into a human antigen binding fragment (Fab) framework.
Next, they introduce a few mutations into its genetic code — perhaps by inserting a sequence from another enzyme with different desirable properties.
A dual luciferase reporter plasmid analogous to the psiCH2-16T reporter used in Figure 2 was constructed by inserting target sequence derived from the cyclinE1 mRNA 3'UTR.
All the inserted sequences were confirmed by sequencing.
Plasmids were retrieved from selected clones and the inserted sequence confirmed by DNA sequencing before transfer to C. crescentus JS4022 by electroporation.
Mito-PTEN was generated by inserting PTEN coding sequence (CDS) into the pCMV/ myc/mito vector purchased from Invitrogen, Life Technologies.
Myc-tagged forms of Hes1 and Hes6 were obtained by inserting the corresponding cDNA encoding sequence from previous vectors into the NcoI/EcoRI and NcoI/StuI sites of the pCS2+MT vector.
ER-GFP plasmid was made from pEGFP-C2, by inserting a C-terminal KDEL sequence by SDM, and an N-terminal ER-signal sequence from calreticulin, by oligo-annealing between NheI and AgeI restriction sites.
Three representative haplotypes carrying alternate MYH9 alleles were generated by inserting the alleles in genomic sequence extending from exon 2 to exon 41, inclusive; the haplotypes were the most common YRI haplotypes consistent with protective (two haplotypes) and susceptible (one haplotype) MYH9 haplotypes from our analysis (see Supplementary Material, Figs S1 and S2).
To construct the recombinant yeast strain for expression of BLIP, an expression vector was constructed by inserting the blip gene and its terminator sequence from S. clavuligerus into the pPICZαA at the region downstream the AOX1 promoter and α-factor mating signal sequence (Fig. 1a, b).
Codon alignments were created from the amino acid alignments by inserting three-nucleotide gaps into corresponding DNA sequences.
