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29 It was designed by inserting an optimized truncated intron sequence into sites within HGF cDNA so that both isoforms of HGF protein are expressed simultaneously and efficiently.
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The interaction was modeled by taking the geometry-optimized DNA oligonucleotide and after inserting an optimized dimer of cyclam ligands into the minor groove, the resulting DNA-dimer complex was then subjected to geometry optimization.
Based on a (HLL 9H design comprising of quarter-waves of high-index material and half-waves of low-index material, we obtain an optimum MLD coating meeting these requirements by inserting a matching layer being half a quarter-wave of Al2O3 between the initial design and an optimized HfO2 top layer.
Test by inserting a thin knife blade.
Test the cake is cooked by inserting a butter knife.
Test cakes by inserting a wooden toothpick.
Add comments to connecting lines by inserting a Text Box, available from the Insert tab.
Test for doneness by inserting a toothpick in a cupcake.
Plasmid pPML7800 was generated by inserting the codon-optimized H1 HA gene into an expression cassette (pPJV7563) that uses the human cytomegalovirus immediate early promoter.
Given our goal to develop a gene-based vaccine for influenza, we tested this vaccine platform by inserting the codon-optimized cDNA for HA from H1 influenza A/Puerto Rico/8/1934 (A/PR/8/34) into a E1-deleted replication-defective Ad5 vector (Ad-PR-HA).
PD was started by initially inserting a drainage tube.
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