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Anesthetized mice were intubated by inserting a sterile, 20-gauge intravenous cannula through the vocal cords into the trachea.
Cervical secretion samples were obtained by inserting a sterile swab into the cervical canal for 15 s.
Briefly, subgingival plaque samples were obtained by inserting a sterile paper point into a subgingival site for 10 s.
Vaginal samples were collected for the purpose of vaginal microflora status assessment by inserting a sterile cotton-tipped wooden swab into the vagina.
Low Vaginal swabs (VS) were collected by inserting a sterile cotton swab (Sterilin Ltd ,UK) 2 3 cm into the vagina and rotating the swab with a circular motion, leaving it in the vagina for approximately 5 s.
After supragingival debridement, subgingival plaque was collected by inserting a sterile paper point (No. 30) into the pocket until resistance was felt and was kept in place for 30 seconds.
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Since many "lung" microbial species hailed from saliva contamination of the bronchoscope, the investigators refined their collection method by inserting a second sterile scope inside the first.
Schirmer tests of tear production without and with anesthesia were performed by inserting a Schirmer tear test sterile strip (35 mm, Alcon Laboratories , Inc Fort Worth, TX) into the inferior fornix, at the junction of the middle and lateral third of the lower eyelid margin, for 5 minutes with the eyes closed.
Ultrasound-guided amniocentesis was conducted using sterile techniques by inserting a 22 gauge, 1.5-inch spinal needle into the amniotic sac at the gestation days (GD) noted in Fig. 1.
Test by inserting a thin knife blade.
Check the egg is cooked by inserting a skewer.
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