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pcDNA3-HA-UCH L1 C90S and H161D mutants were generated by inserting a specific mutation at the Cys 90 and H161 sites in wild-type pcDNA3-HA-UCH L1 plasmid with the use of the Quik-Change Site Directed Mutagenesis Kit following the manufacturer's instructions (Stratagene).
Verify your site, either by creating a blank HTML file with a certain name (generated by Google), or by inserting a specific meta tag in your site index.
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More precisely, the classical approach is generalized by inserting a new specific loop in the main adaptation loop in order to solve a transient fixed point problem for the mesh solution couple.
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The plasmids for small interfering RNA (siRNA) experiments, generated by inserting an oligonucleotide containing a specific siRNA target sequence directed against Twist1 or a scrambled sequence into the pLKO_TRC005 and pLKO.1 vectors, respectively, were purchased from National RNAi Core Facility (Taipei, Taiwan).
Synthetic biology aims to perform various specific functions in organisms by inserting a designed gene network.
Plasmids targeting specific genes were constructed by inserting a 1 2 kb genomic PCR fragment (using primers pairs from Wormbase [ 55] of the targeted gene sequences, see Additional file 3) into plasmid L4440.
This paper reported the development of fluorescent indicators for Hg2+ in green or red color by inserting a circularly permuted fluorescent protein into a highly mercury-specific repressor.
Test by inserting a thin knife blade.
Test the cake is cooked by inserting a butter knife.
Test cakes by inserting a wooden toothpick.
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