Sentence examples for by inserting a sequence from inspiring English sources

Next, they introduce a few mutations into its genetic code — perhaps by inserting a sequence from another enzyme with different desirable properties.

Improving DNA vaccines by inserting a sequence involving NKG2D receptor-ligand interaction was shown to enhance innate and adaptive immune responses [36].

The pGEX-loxP plasmid was generated by inserting a sequence containing the loxP recombination site and bacterial promoter, for Cre recombination and an antibiotic selection gene, respectively (sequence information is available in The Creator Cloning System Manual, Clontech), into pGEX-4T-1 (atersham) athehEcoRIRI and XhoI sites.

The pTRIPZ-shDNMT1 (pTshDNMT1) vector was constructed by inserting a shRNA sequence directed against DNMT1 into the pTRIPZ-empty (pTctrl) vector (Thermo Scientific).

As a vector for phage surface display, a phagemid pEXHAM1 [ 47] was used which was derived from pSEX81 [ 48] by inserting a DNA sequence encoding a tag that consists of six histidine residues (His6-tag), an amber stop codon (Am), and a c- myc epitope between the scFv and the M13 genegene.

The size of the integrated HSV-BAC-shuttle DNA was designed to exceed the packaging capacity of the HSV virion by inserting a stuffer sequence, so that recombinant HSV genomes are efficiently packaged and propagated only after removal of the BAC and stuffer sequences by FLPe.

(H – K ) 10-nm thick electron cryotomographic slices of E. coli cells expressing engineered protein constructs based on FtsZ(D212A) (see also Figure 1 figure supplements 3,5 and Supplementary file 1, Table B ). (H ) Extending the C-terminal linker of FtsZ by inserting a linker sequence pushes the filaments further away from the IM (distance changed from 16 nm to a somewhat variable 16 21 nm).

Alternative splicing may affect the protein sequence in two ways: (i) by deleting or inserting a sequence and creating long and short isoforms, or (ii) by substituting one segment of the amino acid sequence for another [ 11].

The vector, pET 15b, was constructed by inserting a flexible spacer sequence, a hexa-histadine tag and thrombin cleavable sequence.

The plasmid pT7-D2-GFP was constructed by inserting a T7 promoter sequence, cDNA encoding the 5' 1 170 nt of DENV serotype 2 (DENV2) and the GFP sequence into a pUC18 vector (Figure 1A).

To further confirm the inhibition of NF- κB signaling by HSCARG under physiological conditions, the Cre/loxP system was used to generate HSCARG −/− HCT116 cell line by inserting an extra sequence into the fourth exon and adding further stop codons to disrupt the translation of HSCARG.