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An antisense PCDGF cDNA expression vector was constructed by inserting a partial human PCDGF cDNA (-30 bp to 465 bp) into a pcDNA3.1 mammalian expression vector (Invitrogen) in the antisense orientation.
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All expression constructs described below were generated by inserting a full or partial coding sequence of Eph or Dishevelled genes with five glycine linker into Bra>PsBlSlBaMycNt immediately upstream of the Myc-tag sequence.
Mr. Paterson appeared to have won a partial agreement by inserting a significant portion of his health care cuts into the latest in a series of short-term emergency spending bills, which are difficult to amend.
In particular, we examined expression of a reporter gene that was linked to an enhancer that had been weakened either by partial deletions or by inserting a leaky insulator.
In 1975, Hijikata reported percutaneous nucleotomy by inserting a 7-mm-diameter tube under local anesthesia with partial resection of disc material [ 17].
Test by inserting a thin knife blade.
Test the cake is cooked by inserting a butter knife.
Test cakes by inserting a wooden toothpick.
Add comments to connecting lines by inserting a Text Box, available from the Insert tab.
Test for doneness by inserting a toothpick in a cupcake.
Check for doneness by inserting a knife in the center.
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