Sentence examples for by inserting a modified from inspiring English sources

The destination vectors, pCLXSN-DEST and pDEST-CMSCVpuro, were constructed by inserting a modified cassette containing attR sites and ccdB (Invitrogen) between the XhoI and BamHI sites of pCLXSN, and between the EcoRI and BglII sites of pCMSCVpuro, respectively.

doi: 10.7554/eLife.00800 Image A biosensor for extracellular ammonium made by inserting a modified green fluorescent protein (bottom) into an ammonium transport protein (top) All organisms require nitrogen for their survival.

The pattern by which resistance genes are inherited has proved elusive.Dr Thompson and Dr Rybicki's trick was to insert a modified viral gene into the maize.

All the probes were modified during synthesis by addition of an amino linker arm at the 5′ end or were internally modified by inserting an amine modified thymidine base.

Vogel [55], Tamman and Filchers [56] modified Andrade equation by inserting a constant C. The modified equation is also known as the VTF equation, which is as follows: { ln }eta = A + left( {frac{B}{T + C}} right).

We used a modified technique by inserting a Kirschner wire, followed by cannulated drill and finally the half-pin inserted under C-arm control (Fig. 2).

The 26650 cells are modified by inserting a temperature sensor into the cylindrical hole of the jelly roll and regarding their number of tabs at the positive connection.

In a first experiment an endothelin-A receptor (ETAR) probe is modified by inserting a trivalent linker, attaching an albumin affinity tag and labeling the conjugate with the fluorescent dye Cy 5.5.

For this purpose, a transfer vector based on pMSCV (BD BioSciences Clontech Heidelberg, Germany), which contained a puromycin resistance gene, was modified by inserting a 1.3 kb fragment of the rat promoter for translation elongation factor 1 alpha 1 (Eef1a1).

PB-tk-mRFP was further modified by inserting a puromycin-resistance cassette into the BglII and BamHI sites to obtain a new construct named PB-2R-puro.

For this purpose, a bacterial artificial chromosome (BAC RP23-192A7) conthening thentirere murine Col10a1 gene together with 200 kb flanking sequences was modified by inserting a LacZ-Neo cassette into the second exon of Col10a1 by homologous recombination in E. coli.