Sentence examples for by inserting a genomic from inspiring English sources

PD1/CD2-3' and PD1/CD2-3' were generated from PD1/CD2 by inserting a genomic 1.3 Kb BamHI fragment of PD1, spanning intron 7, exon 8, the 3'UTR and 3' intergenic sequences into the Sal/BstI site immediately 3' of the CD2 gene (Figure 2A).

Therefore, most mammalian V2R constructs were build by inserting a genomic DNA fragment containing the second intron (encompassing amino acid position 68 (2.33, numbering of the relative position within GPCR [55]) to amino acid position 321 (7.49) of the respective mammalian ortholog into the double-tagged human V2R-pcDps expression plasmid keeping the human N and C termini.

The pJ5Ω/HG-vector was constructed by inserting a genomic 920 bp BamHI/EcoRI fragment including intron 2 with splice donor and acceptor sites of the human β-globin gene (provided by F. Grosveld, MRC, London) into the BamHI/EcoRI sites in pJ5Ω supplemented with a 5'-MMTV-promotor and 3'-SV40-polyadenylation site [ 31].

The pUA16 control plasmid was constructed by inserting a XhoI/AvaIII genomic DNA fragment (600 bp), containing S. cerevisiae Ser-tRNAAGA gene amplified by PCR with the following pair of primers 5'-CCGC TCGandGGGAGGATTCCTATATCCTTGAGGAG-3' and 5'-GGCTCGATGCATG CCAGGAAGAAATACACTGC-3', into the multicloning site.

We generated the P csp-2csp-2 gfp csp-2csp-2 gfpt by inserting a csp-2 genomic fusionnt constructg 4175 by sequence upstream of the CSP-2A insertingn aTG codon and the whole csp-2 coding region into the pPD95.79 vector.

Plasmids targeting specific genes were constructed by inserting a 1 2 kb genomic PCR fragment (using primers pairs from Wormbase [ 55] of the targeted gene sequences, see Additional file 3) into plasmid L4440.

This mimic was constructed by disrupting the BVDV amplicon at the TaqMan® probe site by inserting a 295 bp fragment of human genomic DNA.

Myc-tagged TTI2 and tti2 -F328S were expressed from the DED1 promoter in YCplac111 by inserting a Not– SstI fragment amplified from genomic DNA using oligoncleotides 5693-1 and 5693-2 (Table 2) downstream of the DED1 promoter-myc cassette (Hoke et al. 2010).

Myc-tagged LCD1 and RPN3 were expressed from the DED1 promoter in YCplac111, a LEU2 centromeric plasmid, by inserting a NotI- SstI fragment amplified from genomic DNA using oligonucleotides 6486-1/6486-1 and 6518-1/6522-1, respectively, downstream of the DED1 promoter-myc cassette (Hoke et al. 2010).

To assess the function of AP-2ɛ in olfaction, we generated a null allele (the "AK" allele) by inserting a Cre recombinase transgene into the endogenous AP-2ɛ genomic locus.

Bollgard II® cotton was produced by inserting a cry2Ab gene (together with an antibiotic marker gene) into the genomic DNA of INGARD® cotton (APVMA 2003).