Sentence examples for by inserting a fragment from inspiring English sources

pAAV2neo was constructed by inserting a fragment containing two inverted terminal repeats (ITRs) from AAV2, the CMV promoter, multi-cloning sites, and BGH polyA into pSV2neo [18].

The RNAi vector of OsDR10 was constructed by inserting a fragment of cDNA digested from cDNA clone BI71N2 using restriction enzymes KpnI and BamHI into the pDS1301 vector (Figure S2B) [37].

The strain MK-ER-Ndt80 (inducible Ndt80 by estradiol, see Carlile et al [ 29]), was made by inserting a fragment of the plasmid PKB80 into the ura3 locus (selecting for Ura+ transformants).

A luciferase reporter construct, psi-FGFR1, was constructed by inserting a fragment of FGFR1 3′-UTR that included miR-133a/b and miR-214 binding sites into the luciferase reporter vector psi-CHECK-2.

The h TERC expression vector pSSP-hTERC was constructed by inserting a fragment containing h TERC in the pSSP plasmid (Salzano et al. 2009), carrying the puromycin resistance gene, under the control of the U1 promoter.

To test in vitro whether the methylation of the P2 promoter affects IGF1 gene expression, we used a reporter gene construct made by inserting a fragment of IGF1 promotertextendinging from −1,014 to +64 bp of the TSS - thus containing 7 of the studied CGs - into a promoter-less CpG-free luciferase expression plasmid.

A construct for Puf2 gene knockout was generated by inserting a 1001-bp 5' frandent and a 945-bp 3' fragment on either side of a human DHFR expression cassette.

In practice the procedure is carried out by inserting a DNA fragment into a small DNA molecule and then allowing this molecule to replicate inside a simple living cell such as a bacterium.

It was constructed by inserting a DNA fragment encoding the E. coli pelB secretion signal sequence into the pDEST17 plasmid.

pMAL-c2X-HisCT was constructed by inserting a DNA fragment encoding a C-terminal Sup35 (254-685 aa, HisCT) protein into the SalI/NotI site of pYex2T.

The expression plasmid for synaptophysin-CFP was obtained by inserting a PCR fragment corresponding to the coding region of the gene into the pECFP-N1 vector (Clontech).