Sentence examples for by inserting a dna from inspiring English sources
In practice the procedure is carried out by inserting a DNA fragment into a small DNA molecule and then allowing this molecule to replicate inside a simple living cell such as a bacterium.
It was constructed by inserting a DNA fragment encoding the E. coli pelB secretion signal sequence into the pDEST17 plasmid.
pMAL-c2X-HisCT was constructed by inserting a DNA fragment encoding a C-terminal Sup35 (254-685 aa, HisCT) protein into the SalI/NotI site of pYex2T.
The targeting vector was constructed by inserting a DNA fragment coding the ChR2 (a generous gift from Dr. G. Nagel, Universität Würzberg, Würzberg, Germany) fused to the Venus gene (a generous gift from Dr. A. Miyawaki, RIKEN BSI, Wako-shi, Japan) into the XhoI site of the Thy-1.2 vector (Thy-1.2-ChR2V; Fig. 1A).
The lentiviral plasmid expressing YFPQL, pLVX-EIP-YFPQL, was constructed by inserting a DNA fragment encoding YFPQL into pLVX-EF1α-IRES-Puro purchased from Clontech.
As a vector for phage surface display, a phagemid pEXHAM1 [ 47] was used which was derived from pSEX81 [ 48] by inserting a DNA sequence encoding a tag that consists of six histidine residues (His6-tag), an amber stop codon (Am), and a c- myc epitope between the scFv and the M13 genegene.
Therefore, most mammalian V2R constructs were build by inserting a genomic DNA fragment containing the second intron (encompassing amino acid position 68 (2.33, numbering of the relative position within GPCR [55]) to amino acid position 321 (7.49) of the respective mammalian ortholog into the double-tagged human V2R-pcDps expression plasmid keeping the human N and C termini.
Briefly, we first generated an approximately 6-kb plasmid by inserting a lambda DNA fragment into the pCRBlunt vector and subjected it to whole genome amplification (WGA) to erase all modifications.
Cambia is trying to mimic the natural process by inserting a packet of DNA from yeast, bacteria and corn DNA that can only be activated when a rice gene is nearby and active.
A constitutively expressed active form of Notch (pCAG-NICD) was constructed by inserting a 2.6K bp DNA fragment from CNIC [ 50], digested with NotI (Fermentas, St. Leon-Rot, Germany), into pCAGGS.
Recently, a transgenic mouse harboring a single copy of a HAC, termed a trans-mini-chromosomal (TMC) mouse, carrying three continuous non-correlated genes from the human genome was generated by inserting a single BAC DNA from chromosome 21 (Miyamoto et al. 2014).
