The 311 m/z could be used as the measuring ion since it exhibited the best peak purity as demonstrated by injecting increasing amount of a standard mixture spiked on a cell matrix (data not shown).
Acute microinjection experiments in frog oocytes were specifically designed to change the endogenous activities of the enzymes, either by directly injecting increasing amounts of a given enzyme (HK, PGM and UGPase) or by microinjection of a positive allosteric effector (glc-6P for GS).
To test this, we co-injected increasing amounts of Dvl2HA and observed a dramatic reduction in the levels of β-catenin that immunoprecipitated with Nkd1myc with increasing amounts of Dvl2HA (Fig. 5C).
Mice were injected with increasing amounts of EBNA1 protein, sacrificed, and the spleen cells were fused to myeloma cells to produce hybridoma cell lines.
Mice were injected with increasing amounts of EBNA1 protein, and the spleen cells were fused to myeloma cells to generate hybridoma cell lines.
(H ) qPCR measurements of nodal transcript abundance in embryos injected with increasing amounts of the hbox12 mRNA.
Groups of 5 Balb/c mice were injected by the i.p. route with increasing amounts of ricin (0.2, 1, 5, and 25 × LD50) in 0.1 mL sterile 0.9% saline per mouse.
Another helpful measure is desensitization, in which increasing amounts of the antigen are injected over a period of time until the sufferer no longer experiences an allergic response.
Increasing amounts of GOx in running buffer (b) (range 625 amol to 625 pmol) were injected into the system, providing the amount of GOx retained on the membrane.
Samples of 5 μl native AahII (3 ng), increasing amounts of freshly prepared rAahII toxin (4, 5, 6, 8, 10, 15 and 20 ng) and increasing amounts of freeze thawed rAahII toxin (4, 6, 8, and 10 ng) in PBS-containing buffer were individually injected by i.c.v.
