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Non-nucleoside reverse transcriptase inhibitors (NNRTIs) are vital in treating HIV-1 infection by inhibiting reverse transcriptase (RT).
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Expression by fetal villi was confirmed by reverse transcriptase polymerase chain reaction.
Sometimes, by accident, reverse transcriptase goes to work on a regular piece of messenger RNA, and copies that into DNA.
Livers were analyzed by quantitative reverse transcriptase polymerase chain reaction for lipid metabolism-related gene expression.
Relative gene-expression analyses were performed by quantitative reverse transcriptase PCR (qRT-PCR).
RNA was prepared using TRIzol reagent and reverse transcribed by Superscript III reverse transcriptase (Invitrogen, CA).
The isolated RNA was treated with RNase free-DNase I (Promega) and reverse transcribed by Superscript III reverse transcriptase (Invitrogen).
cDNA was synthesized by Superscript III Reverse Transcriptase (Invitrogen Life Technologies).
The inhibitory effect of the dandelion extract on reverse transcriptase activity was assessed by the reverse transcriptase assay kit.
Reverse transcription was performed by thermostable reverse transcriptase (Roche, Indianapolis, IN) at 55°C for one hour.
Total RNA was reverse transcribed with random hexamers by using reverse transcriptase (Invitrogen, Carlsbad, CA, USA).
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