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The Q-TOF mass spectrometer was calibrated by infusing a solution of either NaI containing a small amount of cesium ion dissolved in 50% aqueous isopropanol (0.2 μg/μl) or Glu-fibrinopeptide B (1 pmol/μl) dissolved in 30% aqueous acetonitrile containing 0.2% formic acid.
The ESI source was operated in the positive ion mode with the following operating conditions optimized for miltefosine by infusing a solution (2 mg/mL reference standard in methanol/water; v/v; 1 1) using the syringe pump at a flow rate of 2.5 μL/min; transfer capillary temperature 220°C, spray voltage 4.55 kV, capillary voltage 3.29 V, and sheath gas flow 30 (arbitrary units).
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The purpose of this study was to determine whether umbilical lysine uptake can be increased by infusing a lysine solution that does not contain any other amino acid.
The purpose of the present study was to determine whether umbilical THR uptake can be increased by infusing a THR solution that does not contain any other amino acids.
All the parameters were optimized by infusing a standard solution of isopimpinelline (0.1 g L-1) in mobile phase [water + acetic acid 0.1% / methanol + acetic acid 0.1% (90/10)] at a flow rate of 5 μl min-1.
This design also enabled the lysis of each subset contained within a bed for downstream analysis, such as mRNA expression, by infusing a lysis solution through the common inlet and lysate collection from the four separate outlets.
Ionization and MS-MS conditions were optimized by infusing a 0.5-mg/L solution of each sulfonylurea herbicide into the ion source in a 50 50 acetonitrile:water solution with a syringe pump.
IVGTTs were performed by infusing a 20% dextrose solution at a dose of 0.5 g/kg up to a maximum of 35 g.
In order to control for other effects of large-volume resuscitation (e.g. cell swelling), we next increased serum Cl- concentration by infusing a dilute HCl solution into rats with sepsis induced by cecal ligation and puncture [ 46].
EVLW was determined by infusing a 10 ml bolus of saline solution at <8°C via the central venous catheter.
Systemic ionized calcium and sodium levels were maintained stable by infusing a custom-made sodium-free calcium replacement solution.
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