We detect PCSTE3 by indirect immunofluorescence using antibodies designed to extracellular domains of the receptor in yeast expressing the protein.
Anti-rods/rings in patient sera were detected by indirect immunofluorescence, using NOVA Lite HEp-2 ANA substrate (INOVA Diagnostics, San Diego, CA: 508100) as previously described [10].
Changes in CREB phosphorylation in the injected cells were monitored by indirect immunofluorescence using an affinity-purified antiserum (Ab5322) which specifically recognizes CREB phosphorylated at Ser-133, and changes in transcriptional activity of CREB were monitored by expression of a reporter gene regulated by cAMP.
Cells undergoing apoptosis were deteced by indirect immunofluorescence using an antibody against active caspase-3.
ND10 structure can be shown by indirect immunofluorescence using anti-PML, -Daxx, and -SP100 antibodies.
BrUTP incorporated into nascent RNA was detected by indirect immunofluorescence using a monoclonal anti-BrUTP antibody (MAB3424, Chemicon).
After verifying that all mutant PB2 proteins were expressed correctly (Fig. S1), their nuclear import in HeLa cells was tested by indirect immunofluorescence using PB2-specific antibodies.
The level of hPrP and mPrP expression was checked by indirect immunofluorescence using 4F2 and SAF83 mAb, respectively (from Dr. Grassi, CEA, France).
Cell phenotype was studied by optical microscopy and indirect immunofluorescence, using smooth muscle α-actin, desmin and SM-Myosin Heavy Chain (SM-MHC) antibodies.
Z-stacks of GFP-expressing invading cells were obtained by indirect immunofluorescence microscopy using a Zeiss LSM710 confocal microscope.
To see if Nullbasic similarly localizes to the cytoplasm, HeLa cells expressing Nullbasic or the wild type Tat-FLAG fusion protein were visualized by indirect immunofluorescence microscopy using anti-FLAG antibody.
