Moreover most of the research laboratories and centers are equipped with fluorescence microscopes, so indirect fluorescence using Quantum dots can be used for any active targeting nanocarriers (polymers, ceramics, metals, etc).
Visualization of bound antibodies was performed with indirect fluorescence using Alexa 488 and Alexa 568 (Molecular Probes Inc., Eugene, OR, USA).
Distribution of the heterologous glucoamylase α-agglutinin fusion protein on the yeast cell was analyzed by indirect fluorescence microscopy usinglucoamylase α-agglutinintibody.
Reading of the test results was done by indirect immune fluorescence using monoclonal antibodies followed by goat anti-mouse FITC-conjugated IgG (Dianova, Hamburg) [ 30].
MAbs against IL-8 receptors (CXCR1 and CXCR2) were used by indirect fluorescence developed with a rabbit anti-mouse antiserum tagged with FITC (Jackson ImmunoResearch Labs, West Grove, PA).
Detection of oligosaccharide epitopes on the surface of exponential growing cells was carried out by indirect fluorescence as previously described [41].
Having shown by indirect fluorescence methods that undecylimidazole can localize RNA oligonucleotides to membranes, we then attempted to directly visualize RNA membrane association by microscopy.
However, substantial cross-reactivity between B. henselae and B. clarridgeiae detected by indirect fluorescence antibody assay was noted in human sera in a recent study from Japan (2 ).
hCAREx8 expression was evaluated by immunocytochemistry (red fluorescence) using fluorescence microscopy and Western blot.
Fluorescence intensity of the BiFC signal was measured by selecting cells positive for both Nef and Flag-tagged MHC-I, then subtracting the background fluorescence using ImageJ55,56.
