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*Note calculations of non-synonymous polymorphism correct reading frame caused by indels in coding region for comparison to other sequences.
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One of the most common genetic diseases in humans, cystic fibrosis, is frequently caused by various indels in coding regions of the CFTR gene [ 3].
Accordingly, certain patterns of interest in comparative genomics—such as the length distributions of short indels in coding regions can be strongly skewed by sequencing error.
The occurrences of InDels in coding regions of genes were examined by blasting the flanking sequences of 100 bp for each side of the InDel against the tomato ITAG2.3_cds.fasta downloaded from SGN using a PERL script.
We also found 1505 short indels in coding exons in DT40.
Only non-synonymous single nucleotide variants and indels in coding exons and splicing sites were included.
Moreover, the performance of conventional tools of gene prediction and annotation are impaired by indels in protein-coding regions (Hoff, 2009).
Indels caused by IILs in coding sequences are more likely to be selected against.
Nevertheless, no unique SNPs, and only one unique indel, occur in coding sequence in the 2 Mb region flanked by the markers inl and cen-V (Table 1).
Therefore, gain or loss of nucleotides was also frequent in other S. agalactiae strains, but most indels in coding sequences were probably eliminated by purifying selection.
Third, we examined the length-distributions of LS indels in coding regions, considering (a) all LS indels in the ENCODE regions and (b) only those that are supported by the comparative-grade ENCODE sequences.
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