Sentence examples for by incubation with normal from inspiring English sources

Nonspecific binding was blocked by incubation with normal goat serum (Invitrogen) for 30 min at room temperature, and then sections were incubated with anti-Nestin antibody (1 : 300 dilution) for 1 h at room temperature.

Blocking of non-specific antibody binding was performed by incubation with normal goat serum at 37 °C.

Negative controls were carried out by omission of the primary antibodies or by incubation with normal mouse or rabbit IgG (data not shown).

IFI16 was not precipitated by incubation with normal mouse IgG.

Neither the GR nor IFI16 was precipitated by incubation with normal rabbit or normal mouse IgG.

Negative controls were obtained by omission of the primary antibody, and by incubation with normal rabbit IgG for total AKT.

Endogenous peroxidase activity was blocked with 0.3%H2O22 for 10 min and nonspecific binding was blocked by incubation with 10% normal horse serum for 30 min.

After rinsing in 0.1 M phosphate-buffered saline (PBS; pH 7.4), non-specific binding sites were blocked by incubation with 10% normal goat serum for 30 min.

Non-specific antibody binding was blocked for 20 minutes by incubation with 10% normal rabbit serum.

The sections were digested by 10 mg/ml hyaluronidase for 20 min. Nonspecific protein binding was blocked by incubation with 10% normal goat serum.

After digestion with a solution of 0.25% pepsin into 0,1 M HCl (Sigma-Aldrich) for granulocytes or with 0.1% Trypsin (BDH Chemicals) into PBS for macrophages, non-specific binding was blocked by incubation with 5% normal goat serum (Dako Cytomation) within phosphate buffered saline (PBS).