Sentence examples for by incubating two additional from inspiring English sources

Negative controls were generated by incubating two additional sections in diluent only, but mirrored all sequential steps of the two positive sections.

The culture was diluted 1 10 in fresh 3.7% BHI and incubated for two additional hours to reach log-phase growth.

Supernatant was then removed and replaced by 1 ml of complete DMEM containing either 200, 400, or 800 μM ribavirin or favipiravir and the cells were incubated for two additional hours at 37°C and 10% CO2.

The same plate was incubated for two additional weeks, after which these plants were sacrificed for fln1-1 and fln2-1 genotyping.

Infected cells were incubated at 37°C for 30 min. Gentamycin (400 µg/ml) was added and cells were incubated for one additional hour at 37°C.

For Group 1, control cells were incubated for five additional days under normal culture conditions.

Coverslips were then washed with PBS, incubated in freshly prepared 0.2% sodium borohydride in PBS for 20 min, followed by two additional PBS washes.

This is supported by two additional considerations.

Protein was induced by incubating with 0.25 mmol/L IPTG for an additional 24 h.

Copper retention was measured by incubating cells with copper for 24 h, washing, and incubating for an additional 2 8 h in basal media.

To determine the lignolytic activity solely by bacteria, eight additional soil microcosms were prepared with 13C-lignin amended mineral soil and incubated with or without weekly additions of two fungicides: 1 mg·g−1 soil cylcoheximide plus 1.2 mg·g−1 soil fungizone (amphotericin B).