Sentence examples for by incubating the sample from inspiring English sources

Following two final washes in TE (10 mM Tris-HCl pH 8.0, 1 mM EDTA), the immunocomplexes were finally eluted in 150 µl of TE/1% SDS buffer (10 mM Tris-HCl pH 8.0, 5 mM EDTA, 1% SDS) by incubation at 65°C for 15 min. The formaldehyde cross-link was reversed by incubating the sample at 65°C overnight; the input sample was processed in parallel with ChIP samples from this point on.

The hexamers were annealed by incubating the sample at 70°C for 5 min and then immediately quenched on ice.

This effect was minimised by incubating the sample on ice.

The Proteinase K was then inactivated by incubating the sample at 80°C for 15 minutes the next day.

Antigen retrieval was performed by incubating the sample for 20 min at room temperature in proteinase K-PBS.

The ethanol phase from the supernatant was eliminated by incubating the sample at 50°C for 18 hr.

In vitro degradation studies of scaffolds were performed by incubating the samples in phosphate buffered saline (PBS) at 37 °C and pH 7.4 for 30 days.

The resulting tissues were washed with 8% sucrose in water for two hours at 4 °C, dehydrated with an ethanol series, and infiltrated with Eponate 812 by incubating the samples at room temperature for several hours to overnight in increasing concentrations of resin.

They exploited A. clavatus for LDPE degradation by incubating the samples for 90 days and evaluated the biodegradation by means of CO2 evolution, SEM and AFM analysis.

The activity at different temperatures was determined by incubating the samples for 35 min at different temperatures, and stopping them by adding stop solution, then letting the samples cool before the absorbance (420 nm) was measured.

The as-spun nanofibers were crystallized by incubating the samples in 100%, 70 %, 50, and 0% of ethandl for 10%min each, and samples were frofethanolkept for lyophilization overnight.