Sentence examples for by incubating samples for from inspiring English sources

After incubation for 1.5 h at 46°C, the reaction was terminated by incubating samples for 15 min at 70°C.

After incubation for 1.5 h at 46°C, the reaction was terminated by incubating samples for 15 min at 70°C.

For isolation of leukocytes, peripheral blood of PPARα WT and KO mice was obtained by retro-orbital bleeding under isoflurane anesthesia, red cells were cleared by incubating samples for 30 minutes on ice in red blood cell lysis buffer (Sigma-Aldrich, St . Louis MO).

Red blood cells were eliminated by incubating samples for 1 min at room temperature in a red blood cell lysis buffer (Sigma Aldrich).

Endogenous peroxidase quenching treatment was done by incubating samples for 10 min at room temperature in absolute methanol containing 1% hydrogen peroxide.

The oxidation rates of Cp149 dimer and capsid were measured by incubating samples for various times, quenching aliquots with iodoacetamide, and separating disulfide-cross-linked dimers from monomers by nonreducing SDS PAGE.

PCR reactions were performed with a Veriti® Thermocycler (Applied Biosystems) by incubating samples at 94°C for 7', followed by 45 cycles of 95°C for 1', 55°C for 1', 72°C for 1'.

Proteinase K was inactivated by incubating samples at 80°C for 1 hr following incubation at 55°C for 2 hrs.

DNaseI was inactivated by incubating samples at 65°C for 10 min. The sample (12 μl) was then used for reverse-transcription using random hexamers according to the manufacturer's instructions (SuperScript III RT-PCR system, Invitrogen).

The degradation characteristics of the forage species were measured by incubating samples in vitro for up to 96 h in tubes with rumen inoculum.

Digestion was carried out by incubating samples at 55 °C for 16 h with gentle agitation.