Sentence examples for by incubating each sample from inspiring English sources

Proteins were eluted from the beads by incubating each sample with 100 µL of 2 mM biotin in SB17T for five minutes with shaking.

Proteins in the sample were labeled with both biotin and a fluorophore by incubating each sample in 100 µL of 1 mM EZ Link NHS-PEO4-biotin (Pierce), 0.25 mM NHS-Alexa-647 (Invitrogen) in SB17T for five minutes with shaking.

Evans blue dye was extracted from the tissue by incubating each sample in 120  μl formamide (Sigma, St . Louis MO, USA) for 18 h at 70 °C.

Measurements of release kinetics were performed by incubating each sample separately in 2 mL of water in a 24-well plate at room temperature.

Captured FH-WDR5 complexes were then eluted from the FLAG-M2 agarose by incubating each sample in 500 μl of FLAG Elution Buffer (50 mM Tris HCl pH 7.5, 250 mM NaCl, 0.5% NP-40, 0.1% Sodium Deoxycholate) and 0.5 μg/ml FLAG Peptide (PAN Facility, Stanford University) for 25 min at 4°C on rotation.

The hexamers were annealed by incubating the sample at 70°C for 5 min and then immediately quenched on ice.

This effect was minimised by incubating the sample on ice.

The ethanol phase from the supernatant was eliminated by incubating the sample at 50°C for 18 hr.

Proteins were degraded by incubating the sample in a proteinase K solution at 45°C for a few hours.

Antigen retrieval was performed by incubating the sample for 20 min at room temperature in proteinase K-PBS.

The Proteinase K was then inactivated by incubating the sample at 80°C for 15 minutes the next day.