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Highly ordered structures of TMV imprints are produced by increasing the template surface concentration on the stamp which leads to an increase in sensor response by about a factor of 4. The resulting frequency shifts arise from the incorporation of virus clusters into the material.
This inefficiency can be compensated partially by increasing the template concentration in the injection mix.
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Firstly, the number of cycles during PCR should be reduced to prevent undesired mutations; the resultant lower product yield can be offset by increasing the starting template concentration.
The effects of CdTe QDs on PCR did not alter by increasing the concentration of template or primers, but were completely reversed by increasing the concentration of Taq polymerase in the PCR mixture.
As discussed in [13], separation performance can be improved by increasing the number of templates for each note.
The proportion of nontarget products was reduced by increasing the dilution ratio of the template in the next PCR amplification.
However, we were able to enhance the discrimination of KOD exo- by increasing the steric crowding of the template binding site in close vicinity to the residues bearing the C or 5mC of interest.
This may compound problems associated with amplification of low copy number templates by increasing the carryover of potential inhibitors into downstream analysis methods such as qPCR.
The results showed that the favorable phase crystallinity was improved by increasing the hydrothermal synthesis temperature and organic template content, whereas increasing the water content in synthesis mixture decreased the crystallinity.
We are currently improving the performance of the method for hard template-based targets by increasing the accuracy of model ranking and integrating ab initio modeling with TBM to enhance model generation.
Structured templates can provide cues that can improve record quality by increasing the amount of information captured and encouraging consistency.
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