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Secondary metabolism may participate in the desiccation tolerance of M. flabellifolia as indicated by increases in transcript abundance of genes involved in isopentenyl diphosphate biosynthesis.
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The early responses of the transcriptome to both N and P were dominated by increases in transcripts for PPR proteins.
These initial responses are down-regulated within the first 2 3 weeks and are followed by increases in transcripts associated with immune system processes that affect T cell development and activation as well as those involved in cell cycle control.
The overall increase in CoA content during fasting was not driven by increases in the transcript levels of the PanK genes (Table 2).
The increased accumulation of ω-5 and ω-2 gliadins with higher availability of nitrogen was accompanied by increases in related transcripts, determined using real-time RT PCR and in situ RNA hybridization during grain filling (17 DPA).
The primary role of the glucose pool in tension wood formation is also corroborated by large increases in transcript levels of sucrose synthases SUS1 and SUS2 during tension wood formation [ 27, 28].
Although the reason for the global reduction in mRNA stability following UPF1 depletion remains elusive, our results indicate that altering global mRNA stability homeostasis (in this manner at least) suppresses dosage compensation mediated by increases in X-chromosome transcript stability.
We found that the majority (74%) of the genes induced at least 3-fold by heat shock in the study by Gasch et al. (2000) showed concordant increases in transcript abundance and in nascent RNA synthesis after heat shock, again highlighting the transcriptional activation that contributes to the increased RNA abundance for this class of genes.
Using a self-organizing map algorithm (SOMA) to cluster genes, 64 clusters grouped the majority of steroid genes into a single cluster, highlighted in Figure 2. Thus, a major part of the A. fumigatus early hypoxia response is characterized by increases in ergosterol biosynthesis associated transcripts.
Whole-body samples were characterized by a rapid gain of transcripts and increases in transcript size through 40 million reads, while all other parameters level off after 40 million reads.
DNA methylation within a transcribed sequence has been associated with changing the rate of passage of RNA polymerase through the body of the gene [ 27], raising the possibility that the extensively demethylated gene bodies in our treated cells would be accompanied by increases in the amount of primary transcript.
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