We conducted correlation tests to assess whether estimates of NoTO and SSCD were influenced by incomplete sampling in the family subtrees or by the proportion of halophytic taxa in the family subtrees.
We did not find evidence of a node density effect, and the possible bias introduced by incomplete sampling could be expected to affect in a parallel way all character systems when plotted on the same tree topology, and thus not to introduce any strong bias in our results [ 19, 32, 80].
It is not possible at present to sequence the ssRNA population to saturation even with deep sequencing methods, so we cannot yet rule out that such an organisation is an experimental artefact caused by incomplete sampling of the ssRNA population.
However, we did uncover a range of link strengths, from pairs of individuals that were connected only by virtue of belonging to this group to pairs of individuals who were very close friends, so while our power may have been low it is unlikely that our results are invalidated by incomplete sampling.
The HHS sample was designed to be nationally representative, but this aim can be affected by incomplete sampling frame, imperfect identification of samples households, or non-response.
The smaller number of C-terminal variants could be explained by lack of variation in the C-terminal region or by incomplete sampling of variants by PCR survey.
Of these, 21 patients had incomplete sampling and four patients met exclusion criteria.
In the light of the results obtained with the mitochondrial sequences used here, additional interpretations of the history of African elephants become evident and suggest that the conclusions drawn in previous studies may have been hampered by incomplete sample sets.
It must be noted, that a parsimonious approach might be affected by incomplete taxonomic sampling and errors in the species tree.
Previous attempts at reconstructing the entire history of the sudden oak death (SOD) epidemic in California were limited by: (1) incomplete sampling; (2) the inability to include infestations caused by a single genotype of the pathogen; (3) collapsing of non-spatially contiguous yet genetically similar samples into large meta-samples that confounded the coalescent analyses.
