Trials with a GST fusion expression system driven by the stronger T7 promoter did not improve expression levels (results not shown).
The late flowering of GmGBP1-ox plants in SDs elucidated that GmGBP1 might delay flowering time in SDs through autonomous pathway by improving the expression level of FLC, a key flowering inhibitor factor.
Thus, improving the expression levels of these genes might help improve Chinese cabbage leaf head yield.
With the baculovirus preparation process becoming more convenient, researchers will focus on improving the expression levels of exogenous proteins by changing the baculovirus genome.
Thus, improving the expression levels of recombinant protein in bovine mammary bioreactors is crucial.
Inclusion of a third plasmid expressing a transactivator is known to improve protein expression levels by enhancing promoter activity [ 30] or by increasing transpositional frequency of the element [ 29].
Yields were improved by balancing expression levels of each gene, using a fed-batch cultivation strategy, and adding a solvent to the culture for extracting the product from cells.
This is the first report on improving the expression level of the lipase from R. oryzae by replacing prosequences.
Codon optimization by using frequently used codons in the host is an efficient measure to improve the expression level of heterologous gene.
In addition, the introduction of RGD group further improved gene expression level.
