Sentence examples for by immunomagnetic sorting using from inspiring English sources

Experiments were carried out on spleen, lymph nodes and bone marrow suspensions, or splenic B-cells negatively purified by immunomagnetic sorting using the Dynabeads Mouse CD43 Negative Isolation Kit (Invitrogen) (>85% purity).

CD4+ T-cells were purified from PBMNC of five patients and four healthy blood donors with negative-immunomagnetic sorting using untouched CD4+ T-cell kit according to manufacturers' recommended protocol (Miltenyi, Germany).

CD14 positive cells were isolated by immunomagnetic sorting by using the VARIOMACS technique (Miltenyi Biotec GmbH) according to the manufacturer's procedure and cultured in polystyrene tissue culture plates with a hydrophobic surface (Greiner Bio-One).

Bone marrow mesenchymal stem cells were obtained by immunomagnetic sorting of mouse bone marrow cells, using antibodies recognizing the specific markers STRO-1 and c-kit (Myilteny Biotech).

Monocytes were isolated from PBMC by positive immunomagnetic cell sorting using CD14 Microbeads (Miltenyi Biotec, Auburn, CA) which yielded >90% purity when assayed by flow cytometry (FACSCalibur; BD Biosciences, San Jose, CA) using anti-CD14-FITC (BD Pharmingen, San Diego, CA).

Cells expressing NGFR were isolated by immunomagnetic sorting (Dynabeads).

TK-positive lymphocytes were selected after transduction to >90% purity by immunomagnetic sorting for ΔLNGFR expression, and stored frozen up to the day of infusion.

The amount of G-MDSCs and M-MDSCs isolated from NB mice by immunomagnetic sorting was significantly higher compared with that isolated from TF mice.

As briefly illustrated in Tables 1 and 2, there are many combinations of cell-surface markers that can be used to select MSCs from mixtures of cells, which are often performed by cell sorting using FACS [ 30] or immunomagnetic [ 31] methods, although many studies use plastic-adherent stromal cells that are subsequently classified using immunofluorescent phenotyping or flow cytometry [ 5, 32].

Active sorting using acoustic forces can be improved by combining it with passive sorting methods.

Cholangiocytes were further purified by immunomagnetic isolation using antibodies against the cholangiocyte-specific receptor HEA 125 (Progen, Heidelberg, Germany).