Sentence examples for by immunomagnetic selection using from inspiring English sources

Monocytes were enriched from the PBMCs by immunomagnetic selection using anti-CD14 microbeads (CliniMACS, Miltenyi Biotec).

Endothelial cells were isolated from this fraction by immunomagnetic selection using a mouse anti-CD31 antibody (Dako, Baar, Switzerland), followed by an anti-mouse IgG Dynabead, 30 min incubation with each and magnetic separation was performed on MACS columns.

Monocytes were isolated from PBMCs obtained from healthy donor buffy coats by immunomagnetic selection using CD14 microbeads (MACS monocyte isolation kit from MiltenyiBiotec, Auburn, CA), according to the manufacturer's instructions.

Monocytes, T and B cells were isolated from PBMCs by an immunomagnetic selection using MACs microbeads (Miltenyi Biotec GmbH, Bergisch Gladbach, Germany), according to the manufacturer's instructions.

For eosinophil separation the remaining pellet after histopaque centrifugation underwent red cell lysis followed by negative immunomagnetic selection using anti-CD16 coated immunomagnetic beads.

Eosinophils were separated from predominating neutrophils by negative immunomagnetic selection using anti-CD16-coated magnetic beads (Miltenyi Biotec Inc., Auburn, CA, USA).

We wash mononuclear cells twice with Dulbecco's phosphate-buffered saline (PBS, Invitrogen Corporation, Carlsbad, CA), and CD34+ hematopoietic progenitors are purified by positive immunomagnetic selection using the MACS cell isolation system (Miltenyi Biotec, Auburn, CA).

Bone marrow (BM) aspirates were collected at the time of surgery and the presence of DTC was determined by two immunological methods; immunomagnetic selection (using an anti-EpCAM antibody) and immunocytochemistry (using a pan-cytokeratin antibody).

In our patient cohort, the presence of DTCs analysed by immunocytochemistry (using an anti-cytokeratin antibody) and immunomagnetic selection (using an anti-EpCAM antibody) was an adverse prognostic factor (Flatmark et al, 2011).

CD34+ cells were enriched through positive immunomagnetic selection using the CD34 MicroBead Kit (Miltenyi Biotec Inc).

The isolated mononuclear cells then underwent negative immunomagnetic selection using a B Cell Isolation Kit II (Miltenyi Biotec, Bergisch Gladbach, Germany) for purifying B-linage cells.