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The extracellular localization of AMPs in transgenic plants was confirmed by immunohistochemical methods using sections prepared from leaves and roots of transgenic plants.
Twenty-two paraffin-embedded samples were analyzed by immunohistochemical methods using Maspin, Bax and CD34 antibodies.
Thirty-one paraffin-embedded samples were analyzed by immunohistochemical methods using HSP27 and HSP72 monoclonal antibodies.
The cell kinetics of 54 colorectal tumours were examined by immunohistochemical methods, using the monoclonal antibody DNA polymerase alpha which reacts with an antigen found only in proliferating cells.
Miyamoto et al. also reported that patients with positive DPD showed a slightly higher response rate and longer survival than those with negative DPD, but without statistical significance, when DPD expression was evaluated by immunohistochemical methods using antirecombinant human polyclonal antibody (Miyamoto et al, 2000).
The presence of MHC class I antigens and TAP-1 was studied in a series of 93 resection specimens of non-small-cell lung carcinomas (NSCLCs) by immunohistochemical methods using antibodies against the assembled class I molecule, beta 2-microglobulin (beta 2-m), heavy-chain A locus, A2 allele and TAP-1 protein.
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Expression of alpha-1-antitrypsin (AAT) in tumour cells of 102 surgically resected lung adenocarcinomas was examined by immunohistochemical method using anti-AAT antiserum.
S-1 showed antitumour effects in terms of response and survival, regardless of the expression status of TS, when TS expression was evaluated by immunohistochemical method using antirecombinant human TS polyclonal antibody (Miyamoto et al, 2000).
In the present study the anti-tumour effect of the PLGA-based formulations against 101/8 glioblastoma was assessed by histological and immunohistochemical methods using the same tumour model but a more intensive treatment regimen: 3×2.5 mg/kg instead of the previously used 3×1.5 mg/kg.
Ki-67 status was assessed in a central laboratory on paraffin sections by an immunohistochemical method using Clone MIB 1 antibody (DakoCytomation, Glostrup, Denmark).
Details on the immunohistochemical methods used were previously described by Hannemann et al. [ 15].
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by immunohistochemical analyses using
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