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Moreover, by immunohistochemical analysis using a total of 24 serous type ovarian cancer surgical specimens (stage III: n = 21, stage IV: n = 7), excluding samples used for GeneChip analysis, the Kaplan Meier survival curve showed a clear relationship between IDO staining patterns and overall survival (log-rank test: p = 0.0001).
GFP epithelial expression was detected by immunohistochemical analysis using antibodies to GFP or by direct fluorescence (Figure S1A F).
After in situ hybridization, BrdU incorporation was visualized by immunohistochemical analysis using an anti-BrdU antibody (1∶500; Becton Dickinson) and biotinylated anti-mouse secondary antibodies (1∶200).
Similarly, HB-19 and 5-FU treatment significantly inhibited tumor vascularization compared to the untreated tumors as revealed by immunohistochemical analysis using antibodies against the CD31 endothelial marker (Figure 9).
To confirm the finding obtained by ELISA, we examined the amount of amyloid plaques by immunohistochemical analysis using anti-Abeta antibody 82E1, which specifically recognizes Abeta but not APP.
Consistently, no difference was observed between vehicle and S14G-HN treated mouse brains by immunohistochemical analysis using anti-phosphorylated tau antibody AT8 (Fig. 7C, D) and AT180 (Fig.7E, F).
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The production of oxidative-stress related moxidative-stress related detected by the imoleculesocheminal analysis using anthe8-OHdG polungonal antibody (Japan Institute for the Control of Aging, Shizuoka, Japan; n = 5 in each group) using the HistoMouse-Plus kit (Zymed Laboratories, San Francisco, CA, USA).
Furthermore, we examined the level of LAPTM5 protein in NB tumor sections by conducting an immunohistochemical analysis using a specific antibody and confirmed that the protein is highly expressed in hematopoietic cells as reported [23] (Figure 1F).
The relative level of Mecp2 expression in the male and female 'Non-Rescue' and 'Rescue' cohorts was assessed between 3 5 months post- Mecp2 reactivation in males, and 5 9 months post- Mecp2 reactivation in females by immunoblot and immunohistochemical analysis using age and gender-matched wild-type mice as references.
Furthermore, to determine whether synoviolin is induced in the activated HSCs of human cirrhosis patients, we analyzed the co-localization of synoviolin and α-SMA in healthy and cirrhotic liver sections by double-labeled fluorescent immunohistochemical analysis using a liver human tissue array (Fig. 1E).
In order to test whether the decrease in the iron levels in H/I aTf led to a decrease in lipid peroxidation by radical oxygen species, an immunohistochemical analysis using anti-HNE antibody (which recognizes Nα-acetyllysine-HNE fluorophore) a lipid peroxidation product was carried out.
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by immunohistochemical analysis demonstrating
by spectrophotometric analysis using
by multivariate analysis using
by immunohistochemical detection using
by densitometry analysis using
by bioinformatic analysis using
by quantitative analysis using
by immunohistochemical method using
by elemental analysis using
by biochemical analysis using
by Western analysis using
by Probit analysis using
by morphodensitometric analysis using
by thermal analysis using
by defect analysis using
by microsatellite analysis using
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