Sentence examples for by immunofluorescent staining using from inspiring English sources

The characteristics of the proliferating cells were examined by immunofluorescent staining using antibodies against deltaNP63, ABCG2 and Keratin 3/12.

b Macrophages loaded with red fluorescent nanospheres infiltrating the brain metastases detected by immunofluorescent staining using anti-human CD68 antibodies labeled with APEX Pacific Blue fluorochrome.

Nerve fibers in matrigel were detected by immunofluorescent staining using antibodies to nerve marker proteins GAP43 (Abcam, cat # ab12274), neurofilaments 200 (Sigma, cat # 4142), peripherin (Abcam, cat # 4666) and tyrosine hydroxylase (Abcam, cat # 112).

First, to demonstrate the doxycycline-dependent induction of E-selectin expression, ES-Endo cells were incubated with increasing concentrations of doxycycline (0 2000 ng/ml) for 5 hours, and the E-selectin expression level on the plasma membrane was analyzed by immunofluorescent staining using anti-E-selectin antibody.

Increased expression of PCAF in LNCaP cells was further confirmed by immunofluorescent staining using an antibody to PCAF.

Localization of the HA- or GFP-tagged proteins was then determined by immunofluorescent staining using the appropriate antibodies.

The reactivity of anti-dsDNA antibody was tested by immunofluorescent stain using the Crithidia luciliae test (binding site) with mice sera at a dilution of 1 40 in PBS, as suggested by the manufacturer.

Cytofluorimetric sorting of the isolated SCA-1+ cells was carried out by double immunofluorescent staining, using the following directly labeled antibodies (obtained from Pharmingen, San Diego, CA): PE-SCA-1/Ly-6A/E (clonE13-161.7.7), R-APC-CD45R/B220 (clone RA3-6B2), R-APC-CD4/L3T4 (clone GK 1.5), R-APC-CD8a/Ly-2 53-6.7 53-6.7) and R-APC-CD11b (clone M1/70).

Apoptotic cells were identified by fluorescent double immunofluorescent staining using caspase-3 and terminal deoxyuridine nick-end labelling (TUNEL).

Binding of ApoPep-1 to OA joints was demonstrated by ex vivo imaging and immunofluorescent staining using TUNEL and histone H1 and type II collagen antibodies.

Ceramide was also assessed via immunofluorescent staining using an anti-ceramide antibody.