Sentence examples for by immunofluorescent microscopy using from inspiring English sources

At 60 minutes, the macrophages were fixed and visualized by immunofluorescent microscopy using an anti-B.

After 60 minutes, the macrophages were fixed, permeabilized, and examined by immunofluorescent microscopy using an anti-biotin antibody conjugated to Texas Red (Fig. 3A).

The presence of Ang-(1 12) in the cultured cardiac myocytes were evaluated by immunofluorescent microscopy using a protein A column purified anti-rabbit rat Ang-(1 12) primary antibody (1∶100 dilution).

After three washes with PBS the slides were mounted in Citifluor and examined by immunofluorescent microscopy using a Leica DMRA microscope.

After three washes with PBS the slides were mounted in Citifluor (Agar Scientific, Essex, UK) and examined by immunofluorescent microscopy using a Leica DMRA microscope equipped with a Leica DFC350FX digital camera and Leica Application Suite (LAS) software (all Leica Microsystems, Wetzlar, Germany).

TUNEL-positive cells were visualized by immunofluorescent microscopy and counted using a 20× objective.

In the present study, MC-1 receptors were identified on the cells by immunofluorescent microscopy, Western blotting and MSH binding sites using radiolabelled α-MSH.

Proliferating cells that incorporated BrdU during the labeling period were identified using an anti-BrdU antibody and detected by immunofluorescent microscopy.

(C ) Splenic PNA+ GCs (green, highlighted by red ellipses) were assessed by immunofluorescent microscopy of frozen sections and the numbers of GC/tissue section was calculated using Imaris software.

Hsc70 localization was monitored by immunofluorescent microscopy.

Cells were then analysed by immunofluorescent microscopy and a flow cytometer.