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GDNF expression and EGC apoptosis were determined by immunofluorescence using specimen from CD patients.
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The Ipr1 protein was detected by immunofluorescence using Ipr1-specific monoclonal antibodies (Fig. 2h).
Identification of the virus was confirmed by immunofluorescence using human anti-rubella virus specific hyperimmune serum.
The intracellular localization of CS-HA1 mutants was studied by immunofluorescence using specific antibodies against either CS or HA1.
The subcellular localization of RH5 was determined by immunofluorescence using anti-RH5 rabbit antibodies.
Biopsy specimens were analyzed by the same operator in the same laboratory by means of optical microscopy followed by immunofluorescence using polyclonal antibodies to IgG, IgM, IgA, C3, C1q, kappa, and lambda free light chains.
Cellular localization was analysed by immunofluorescence using standard techniques.
Cells undergoing apoptosis were deteced by indirect immunofluorescence using an antibody against active caspase-3.
ND10 structure can be shown by indirect immunofluorescence using anti-PML, -Daxx, and -SP100 antibodies.
All specimens were then observed for immunofluorescence using Nikon Eclipse Ti-E Epi-Fluorescence microscope with 20X objective lens.
Indirect immunofluorescence using whole cells of B. pertussis has the same disadvantages as complement fixation Oral fluid can be sampled by standardised means, e.g. Oracol swabs (Malvern Medical Developments) or OraSure oral specimen collection devices (OraSure Technologies Inc).
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