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Acanthamoeba in our patient was identified by immunofluorescence testing using rabbit antibodies to Acanthamoeba (1, 5 ).
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Antibodies directed against cell-surface antigens of the immunizing cells of 7 leukaemias were not detectable by immunofluorescence tests using sera from the respective immunized mice.
Etiological diagnosis of HFMD and herpangina has classically been dependent upon isolation of viruses and the identification of these either by neutralization tests using Lim-Benyesh-Melnick (LBM) pools or monospecific antisera as well as by immunofluorescence tests using serotype specific monoclonal antibodies [ 3, 4].
Our approach to establishing susceptibility to infection was to use quantitative PCR supported by immunofluorescence testing.
Blood samples (1 punch ≈15µL/rodent) were eluted in 300 µL of phosphate-buffered saline and tested for antibodies to arenaviruses by indirect immunofluorescence antibody (IFA) testing using Morogoro virus as antigen.
The reactive samples underwent confirmatory immunofluorescence testing by using H5-transfected 293 T cells as the test antigen (13 ).
RSV was detected by immunofluorescence tests and virus isolation.
These patients were tested positive by immunofluorescence test for rabies on fresh brain tissues collected postmortem.
In Australia, routine chlamydia testing using culture or direct immunofluorescence assays that detect elementary bodies by fluorescent microscopy [ 6, 7] started in the 1980s.
Influenza virus isolates were subsequently confirmed by immunofluorescence and typed by hemagglutination-inhibition tests using strain-specific antisera provided by the WHO Collaborating Centre for Influenza at the Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
To detect SSc-associated autoantibodies in a comprehensive way, we used the search strategy commonly performed in diagnostic procedures for connective tissue diseases based on a HEp-2 cell immunofluorescence assay followed by tests using cellular extracts and/or recombinant antigens.
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