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Fos plumes were visualized by immunofluorescence processing, using NDS, goat anti-cfos and donkey anti-goat AlexaFluor 488 (excitation = 488 nm, emission = 519 nm; Molecular Probes-Invitrogen, Carlsbad, CA) as described previously [2], [3], [23], [24], [25], [76].
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(d) PrPSc was detected by immunofluorescence staining using mAb 4H11 (red).
Confirmation of LASV GPC expression was done by immunofluorescence by using 50 μl/ml of 9E9 mAb and by FACS using 10 μg/ml of 4C8 mAb.
Sporozoites were detected by immunofluorescence staining using mouse monoclonal antibodies against CSP (1 2000), anti-mouse Alexa-488 (1:1000), and anti-mouse Alexa 594 (1:1000).
HeLa cells were treated with or without 800 μmol/L H2O2 for 30 min, and then endogenous NEDD8 was examined by immunofluorescence staining using anti-NEDD8 antibody.
Caspase-3 activation was analyzed by immunofluorescence analysis using a specific anti-active caspase-3 antibody.
Lineage specification was followed by immunofluorescence by using specific antibodies.
The extinction of TbPFR2 was followed by immunofluorescence microscopy using the L8C4 anti-TbPFR2 monoclonal antibody.
We also examined DLG3 protein by immunofluorescence microscopy using anti-DLG3 antibodies.
The Ipr1 protein was detected by immunofluorescence using Ipr1-specific monoclonal antibodies (Fig. 2h).
Identification of the virus was confirmed by immunofluorescence using human anti-rubella virus specific hyperimmune serum.
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